DNA Polymerase III Holoenzyme

Pol III Holoenzyme is a big molecule that consists of 10 different subunits. Three subunits alpha, epsilon and theta form the core and there are 2 cores in a Pol III Holo enzyme complex that linked by tau subunit. Each core is responsible for leading and lagging synthesis. The gamma complex (clamp loader) is composed of gamma, delta, delta prime, chi and psi subunits; take in charge in loading beta clamp onto primed DNA. The complex consist of two cores and the gamma complex is called as Polymerase III*. When beta clamp is added to each Continue Reading →

Overview of 5 DNA polymerases in E. coli cell

So far, there are 5 distinct DNA polymerases have been found in E. coli cell: Pol I, Pol II, Pol III, Pol IV, and Pol V. While Pol III (Pol III Holoenzyme) is a primary polymerase involved in DNA replication, the others take part in other processes such as DNA repair, translesion synthesis … rather than in chromosome replication. Pol I is encoded by polA gene and belongs to the A family. During DNA replication, PoI synthesizes the gap between Okazaki fragments to complete lagging product synthesis by its 5′-3′ polymerase activity. Continue Reading →