As you well know, DNA molecules consist of two long polynucleotide chains that coiled around each other to form a double helix. The two strands of DNA run in opposite directions to each other and therefore are antiparallel. While direction of one strand is 3’-5’, the other’s is 5’-3’. However, DNA polymerase can only add free nucleotides to the 3’-OH end of primer, to synthesize new strand in a 5’-3’direction. To solve this problem, replication process on 2 strands is performed in a semi-discontinuous manner. On Continue Reading →