Overview of E. coli single stranded binding protein SSB and its cooperativity.

E. coli single stranded binding protein (Eco SSB) is encoded by ssb gene which consists of 534 bp in coding region. Product of ssb gene contains 177 amino acids with a molecular weight of 18,9 kDa. In solution, Eco SSB forms a tetramer as the active form (sometimes be viewed as a “dimer of dimers”). SSB is known to tightly bind single stranded DNA (ssDNA) with very high affinity and protects that ssDNA from nuclease, reannealing or secondary formation …  or keeps ssDNA intermediates, therefore involved Continue Reading →

Overview of 5 DNA polymerases in E. coli cell

So far, there are 5 distinct DNA polymerases have been found in E. coli cell: Pol I, Pol II, Pol III, Pol IV, and Pol V. While Pol III (Pol III Holoenzyme) is a primary polymerase involved in DNA replication, the others take part in other processes such as DNA repair, translesion synthesis … rather than in chromosome replication. Pol I is encoded by polA gene and belongs to the A family. During DNA replication, PoI synthesizes the gap between Okazaki fragments to complete lagging product synthesis by its 5′-3′ polymerase activity. Continue Reading →