Overview of E. coli single stranded binding protein SSB and its cooperativity.
E. coli single stranded binding protein (Eco SSB) is encoded by ssb gene which consists of 534 bp in coding region. Product of ssb gene contains 177 amino acids with a molecular weight of 18,9 kDa. In solution, Eco SSB forms a tetramer as the active form (sometimes be viewed as a “dimer of dimers”). SSB is known to tightly bind single stranded DNA (ssDNA) with very high affinity and protects that ssDNA from nuclease, reannealing or secondary formation … or keeps ssDNA intermediates, therefore involved Continue Reading →