Based on the polarity of double stranded DNA molecule and characteristic of DNA polymerase III Holoenzyme that can only synthesize DNA in 5′-3′ direction, replication is carried in a discontinuous manner. There are two core polymerase on the replisome and each core takes part in leading or lagging synthesis. On the leading template, the new strand is synthesized continuously in the same direction with the fork progression, while on lagging template, the synthesis occurs in the opposite direction with fork movement. Therefore, the synthesis of lagging Continue Reading →