Replisome is a multiple protein complex that works together on the template in order to synthesize new DNA. Basically,  E. coli replisome contains a hexamer helicase, single stranded binding protein SSB, primase DnaG, 2 Polymerase III core, a gamma complex (also known as clamp loader) and β clamp. Helicase is a homohexamer that encircles the lagging strand like a ring. It translocates along the ssDNA and separate the duplex DNA using energy from ATP hydrolysis. The unwinding of double stranded DNA and discontinuous synthesis of lagging strand leaves Continue Reading →